Asian Journal of Bio Science

B. Satheesh Kumar ¹, M. Krishna Reddy ², M. S. K. Prasad ¹, A. V. N. Appa Rao ³

Affiliations
1 Department of Biochemistry, Kakatiya University, Warangal (A.P.), IN
2 Department of Zoology, University College, Kakatiya University, Warangal (A.P.), IN
3 University College of Pharmaceutical Sciences, Kakatiya University, Warangal (A.P.), IN

Abstract

Progesterone is important for initiation and maintenance of pregnancy (hormone of pregnancy).The discovery that in the absence of progesterone, pregnancy cannot be initiated or maintained provided the basis for a massive effort of synthesis of antiprogestin molecules to develop emergency contraceptive pills. By the consequences, till date few progesterone antagonists (antiprogestins) have been synthesized which bind to and block the progesterone receptor. However, none of the antiprogestins are “pure” antiprogestins, having marked antiglucocorticoid properties as well, especially at higher doses. Thus the search for the “ideal” progesterone antagonist continues. The main objective of the present work is to evaluate antiprogestin activity of progesterone analogue, 14 β- hydroxy progesterone. A pregnane glycoside, carumbelloside-I (3-O-beta-D-glucopyranosyl-(1→6)-beta-Dglucopyranosyl- 3beta,14beta-dihydroxypregn-5-en-20-one) was isolated from the n-butanolic extract of Caralluma umbellata (Asclepiadaceae) and was modified to a structural analog of progesterone, 14 β- Hydroxy progesterone (14β- OHP) in just two simple steps. Anti-implantation and abortifacient activities of the compound have been studied to evaluate its antiprogestin activity. To study the anti-implantation effect, pregnant wistar strain albino rats were administered with 14β-OHP (daily dose of 5 mg/Kg body weight) intraperitoneally from the day 1to day 7 of pregnancy. On day 10, laparotomy was performed under light ether anesthesia. The uteri were examined to determine the number of implantation sites. The abdomens were sutured and the animals were allowed to go on term. The number of young born at term was also recorded. The abortifacient activity was studied in another group of pregnant rats. The animals were treated with 14β- OHP (5 mg/Kg body weight daily) intraperitoneally from day 8 to 12of pregnancy. They were sacrificed on day 19 of pregnancy. Both horns of uterus were observed for the number of live fetuses. The compound showed about 44.23% anti-implantation and 62% of abortifacient effect at their corresponding dose levels tested. The results indicated the antiprogestin activity of 14 β- hydroxy progesterone in albino rats.

Keywords

14β- OHP, Anti-Implantation, Abortifacient, Antiprogestin and Contraceptive.

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R. Vijaya Lakshmi ¹, J. Madhukar ², M. S. K. Prasad ²

Affiliations
1 Department of Biochemistry, NRI Medical College, China Kakani (A.P.), IN
2 Department of Chemistry, Kakatiya University, WARANGAL (A.P.), IN

Abstract

Riboflavin carrier protein (RCP) from pigeon egg white and egg yolk have been isolated. The molecular weight of purified RCP of pigeon egg white and yolk has been found to be nearly same as that of previously isolated hen egg white and yolk RCPs. The amino acid composition of pigeon RCPs were observed to be almost similar to that of hen RCPs.

Keywords

Riboflavin Carrier Protein, Amino Acid Composition, Purification, Columba livia.

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R. Vijaya Lakshmi ¹, J. Madhukar ², M. S. K. Prasad ³

Affiliations
1 Department of Biochemistry, N. R. I. Medical College, CHINA KAKANI, (A.P.), IN
2 Department of Chemistry, Univ. Arts & Science College, Kakatiya University, Warangal, (A.P.), IN
3 Department of Chemistry, Kakatiya University, Warangal, (A.P.), IN

Abstract

Riboflavin carrier protein (RCP) has been purified from both pigeon and hen egg- yolk by ion exchange chromatography and gel filtration techniques. Purity of pigeon egg- yolk and hen egg-yolk RCPs are determined by SDS-PAGE. Their structures are compared using Circular Dichroism (cd) Spectra and Fluorescence Spectra. For Pigeon egg-yolk RCP amino acid composition was analyzed. In the present study it is observed that the two proteins have similar secondary structure but they differ in their tertiary structures. The amino acid composition of pigeon egg-yolk RCP has close similarity with hen egg-yolk RCP. This study suggests the existence of some significant structural difference between RCPs from pigeon and hen.

Keywords

Riboflavin Carrier Protein, Protein Purification and Characterization.

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